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According to bovine liver cells within the PC genome and PCcDNA compared with an intron sequence

Nach Rinderleberzellen innerhalb des PC (Pyruvat-Carboxylase) Genom und PCcDNA verglichen mit einer Intron-Sequenz, wobei Dann einen exogenen DNA-Sequenzen, so dass die endgültige Mutantenfragmenten Länge konstruieren größer ist als die Länge des PCcDNA erfolgreich aufgebaut PCcDNA die Wettbewerbs DNA-Vorlage, und dann die Wettbewerbs PCR-Verfahren von Propionat gelten für in-vitro-Kultur von neugeborenen Rindern Hepatozyten-Monoschichten PCmRNA Ebene. Die Monoschicht-Hepatozyten in Kultur Flüssigkeit Natriumpropionat mbt wien Konzentration jeweils 0, 1,5, 2,5, 3,5 und 4,5 und 8,5, 11,5 mmol \ / L. Innerhalb von 24 Stunden wurde die Gesamt-RNA extrahiert und reverse Transkription und in dem gleichen System mit den gleichen Primern amplifiziert Band und Konkurrenz mit einer Vorlage.

Ziel: Studie über akute progressive Hirninfarkt (akute progressive Hirninfarkt APCI) bei Patienten mit Serum hochempfindliche C-reaktiven Proteins (hs CRP CRP) und peripheren mononukleären Blutzellen (periphere mononukleäre Blutzellen und PBMC CX3C Trend Faktor-Rezeptor 1 (CX3C Chemokin receptor1 CX3CR1) Expression und seine Bedeutung bei der Krankheitsverlauf in verschiedenen mbt schuhe Zeiträumen. Methoden: 91 Patienten mit akutem Hirninfarkt wurden von 24 h gesammelt, davon 28 Fälle von APCI (Progress-Gruppe) und 63 Fälle von akutem Hirninfarkt (stabile Gruppe ). Die Expression von PBMC und CX3CR1 hs-CRP wurde durch Immuntrübungsverfahren detektiert und Durchflusszytometrie in zwei Gruppen von Patienten bei der Aufnahme, siebten, 14 und 30 Tage nach der Aufnahme.